. 2015 Jul 3;6(1-2):1–6. doi: 10.1007/s13317-015-0067-5

Table 2.

Time evolution of two-step strategy for NCA/ANA detection/measurement

Era	Years	Methods
Manual ANA IIF–reflective adding on–manual ENA	1970–2000	Manual IIF Manual monoplex methods Low automated monoplex methods
Manual ANA IIF–reflective adding on–automated ENA	2000–2014	Manual IIF Full automated monoplex methods Low automated multiplex methods
Automated ANA IIF–reflex adding on–automated ENA	2015–…	Automated IIF Full automated monoplex methods Full automated multiplex methods

For the terminology see text