Fig. 2.

Growth of MHM21 in LB medium supplemented with different amounts of anydrotetracycline. Overnight cultures of MHM21 (spvA::[I-SecI site, cat]) were adjusted to OD₆₀₀ = 0.01 in LB medium with 100 µg/ml ampicillin. Two hundred microliters of the culture were transferred to wells of a 96-well plate with different concentrations of AHT (0, 125, 500 and 1,000 ng/ml) with each condition assayed in triplicates. The plate was incubated in a plate reader (Victor³, Perkin Elmer) at 30 °C and the absorbance at 590 nm was measured hourly.