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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: J Immunol. 2015 Mar 16;194(8):3820–3828. doi: 10.4049/jimmunol.1301542

Figure 1. Enhanced ETP and DN2 thymocyte expansion in the presence of IL-18.

Figure 1

A) Gating strategy used for discriminating ETP and DN2 thymocyte subsets. Sort-purified ETP or DN2 thymocytes were co-cultured on OP9-DL4 stromal cells in culture media supplemented with IL-18 or IL-7 alone or in combination and cell yields on day 7 were measured using a hemocytometer. B) Cell number in ETP cultures stimulated with indicated concentrations of IL-7 and IL-18. C) Cell yields in ETP and DN2 cultures stimulated with 5ng/ml IL-7 and 100ng/ml IL-18. Data are presented as mean ± SEM of 3 replicate wells for each experimental condition and are representative of three experiments with similar results. ***p <0.001, **p <0.05.