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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: J Immunol. 2015 Mar 16;194(8):3820–3828. doi: 10.4049/jimmunol.1301542

Figure 2. Enhanced proliferation and survival of ETP in the presence of IL-7 and IL-18.

Figure 2

Sort-purified ETPs were co-cultured with OP9-DL4 stromal cells in media supplemented with IL-18 or IL-7 alone or in combination. A) Survival of cells after 7 days of culture was assessed using Annexin V and 7AAD binding (percentages of cells in each quadrant are represented as the mean ± SEM of 3 replicates wells). B) CSFE dilution profiles for ETPs expanding under indicated conditions on day 3, 4, or 6. Histograms are representative of duplicate samples from each experimental condition and are representative of at least three experiments with similar results. **p<0.05