Fig. 1.

Scheme of experimental strategy. Chromatin of Drosophila control cells or cells stably expressing CENP-A^CID–GFP or H3.3-GFP was digested with MNase and anti-GFP affinity purification was performed on the digested soluble chromatin. After washing steps, proteins were eluted by boiling in Laemmli buffer and separated by SDS-PAGE. Following excision from the gel and in gel tryptic digestion, peptide samples were analyzed by LC–MS/MS. (Adapted from [1].)