Figure 6.

Pathophysiological Role of Glutamate in Insulin Secretion

(A–C) Insulin secretion from pancreatic islets of Wistar (A, n = 8 for each), GK (B, n = 6 for each), and ZF (C, n = 8 for each) rats. The inset in the middle panel (B) is included to magnify the scale of the y axis in GK rats. The concentrations of dm-glutamate used were 10 mM, 10 mM, and 50 mM for Wistar, GK, and ZF rats, respectively.

(D) Production of glutamate isotopomers (M+2 to M+5) by glucose stimulation in pancreatic islets of Wistar, GK, and ZF rats (n = 3 for each).

The data are expressed as means ± SEM. Results are representative of three independent experiments. Dunnett’s method (A–C) and Welch’s t test (D) were used for evaluation of statistical significance. ^∗p < 0.05; ^∗∗p < 0.01; n.s., not significant. See also Figure S6.