Figure 2.
Localization of mRNAs in Granules Does Not Rely on P-Body Formation but Rather Recruits P-Body Components and Coalesce following Glucose Depletion
Epifluorescent images of cells grown to exponential phase and then incubated in either SCD (+glucose) or SC media (−glucose) for 10 min.
(A) Images of wild-type (WT) cells expressing the P-body marker Dcp2p-CFP as well as MS2L-tagged mRNAs visualized with pMS2-GFP3.
(B) Images from mutant strains deficient in P-body formation (edc3Δ lsm4ΔC) also expressing Dcp2p-CFP, MS2-tagged mRNA, and pMS2-GFP3. Scale bar, 2 μm.
(C and D) Bar charts showing (C) the percentage of mRNA granules colocalized with P-bodies (Dcp2p) following glucose starvation and (D) the mean number of mRNA granules per cell in wild-type (WT) and edc3Δ lsm4ΔC mutants under glucose replete and starvation conditions. z stack merged images were used to count granules across 50 cells.
Errors bars are ±SE. See also Figure S2.