Figure 5.
Cycloheximide Treatment Inhibits the Coalescence of mRNA Granules
(A) Schematic representing the culture treatment regimen. Gray and white bars represent 10 min growth in media with or without glucose respectively, and arrowheads denote the point of cycloheximide addition.
(B) Epifluorescent images of cells expressing Dcp2-CFP and MS2L-mRNA/pMS2-GFP3. Cells were grown to exponential phase in media containing glucose and then either (1) treated with cycloheximide for 10 min or (2) treated with cycloheximide for 10 min and then incubated in media lacking glucose for 10 min.
(C) Bar chart depicting quantification of the average mRNA granules per cell for the treatments described above. Merged z stacks were used to count the number of granules in 50 cells.
Errors bars are ±SE. See also Figure S4.