Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 1;29(15):1618–1630. doi: 10.1101/gad.263210.115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Koltowska et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 7. — mafba is downstream from Vegfc and SoxF transcription factors. (A) Expression of mafba at 30 hpf in control uninjected (n=38/41) and MO-sox18/sox7 dMO-injected (n = 24/25) embryos (left) and at 48 hpf in control uninjected (n = 30/33) and MO-sox18/sox7 dMO-injected (n = 18/18) embryos (right). Arrows indicate PCV, and an asterisk indicates reduced expression. Bars, 100 μm. (B) mafba expression at 48 hpf in control and vegfc-induced embryos as well as vegfc-induced embryos injected with MO-sox7, MO-sox18, and MO-sox18/sox7 dMO. Arrows indicate PCV expression, and asterisks indicate reduced/absent expression. Bars, 100 μm. (C) Quantification of mafba expression in embryos from E, with embryos scored as high, medium, and low mafba expressors. Control, 88%, n = 37/42, high; MO-sox7, 89%, n = 39/44, high; MO-sox18, 71%, n = 25/35, medium; dMO, 71%, n = 34/48, low. (D) Quantification of VEC number in vegfc-induced control and MO-sox18/sox7 dMO-injected embryos. Mean ± SEM. Control, n = 12; control vegfc-induced, n = 34; dMO, n = 11; dMO vegfc-induced, n = 25; t-test. (ns) No significant difference. (E) qPCR for sox18 expression in FACS-sorted zebrafish ECs from MO-vegfc, vegfc-induced, and control embryos. egfp served as a control for EC expression levels. Expression is relative to the geometric average of kdrl, cdh5, and lyve1b expression. Mean ± SEM. (F) Working model of Mafba function in lymphangiogenesis: Vegfc up-regulates mafba expression in secondary sprouts and in a Sox18-dependent (redundancy with Sox7) manner. Mafba is essential for PL migration. Vegfc controls venous proliferation independently of Mafba or Sox18/7.