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. 2015 Aug 1;29(15):1661–1675. doi: 10.1101/gad.265876.115

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© 2015 Wang et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Alignment of DNA flanking parS sites. (A) Normalized Hi-C contact matrices of strains with a single origin-proximal parS site at −1° (BDR2996) or +4° (BDR2985). Both strains also contain the weak parS site at +91°. A bar graph of the percentage of interarm interactions compared with total interactions for the indicated strains is shown in B (see Supplemental Fig. S6A for details). (C) Strains harboring the endogenous parS site at +91° but lacking all eight origin-proximal parS sites (parSΔ8) in an otherwise wild-type background (BNS1657) or in cells lacking parB (BWX2761) or parA (BDR3007). (D) A strain (BWX3304) harboring the endogenous parS site at +91° and smcts grown at 30°C and shifted for 20 min to 42°C. (E) A strain (BWX3172) with an ectopic parS site inserted at +122° in an otherwise wild-type background. (F) A strain (BWX3359) harboring an ectopic parS site at −94° and smcts grown at 30°C and shifted for 40 min to 42°C. A 20-min time point was not tested. (G) A strain (BWX3231) with two parS sites inserted to +91° and −94° in parSΔ9. The positions of the relevant parS sites (in degrees) are indicated in the contact maps. Schematic representations of the long-range interactions and the disposition of the chromosome are shown at the right (see also Supplemental Fig. S8A,B).