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. 2015 Sep;21(9):1532–1543. doi: 10.1261/rna.051524.115

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© 2015 Haag et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — NSUN6 binds to a specific subset of tRNAs. (A) The relative abundance of sequence reads from different tRNAs obtained in CRAC experiments using UV or 5-AzaC crosslinking with cells expressing the NSUN6-HisPrcFLAG (NSUN6) protein or control cells (FLAG) is shown graphically. The percentage of hits for all isoacceptors of the listed tRNA species is given. The asterisk (*) marks tRNAs that are not enriched in the NSUN6 samples. This includes, from bottom to top, tRNA^{Met, Leu, Gly, Glu, Ser, Asp, Lys, Ala, Pro, Val}, tRNA pseudogenes, and tRNA^{Ile, Gln, Asn, Tyr, His, Trp, Phe}; the hash (#) indicates tRNA^{His, Trp, Phe}. (B) UV crosslinking and pulldown of the NSUN6 associated RNA was performed as described in Figure 1. The RNA was precipitated from the isolated protein–RNA complexes, separated on a denaturing polyacrylamide gel and transferred to a nylon membrane. Different tRNA probes against the indicated isoacceptors identified in the CRAC experiments were used for Northern blot analysis. Input: 1%, elution: 50%.