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. 2015 Sep;21(9):1566–1577. doi: 10.1261/rna.051466.115

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© 2015 Radak et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 2. — Reaction scheme for (de)protonation of the HDVr at U-1:O2′ and Mg²⁺ binding events needed to attain catalytically active states with and without Mg²⁺ (k_cat^Mg2+ and k_cat, respectively). RH indicates a (neutral) protonated reactant state (O2′H), while R⁻ indicates a deprotonated reactant state (O2′⁻). In all cases, C75 is assumed to be in a protonated state.