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ySMN·Gemin2 forms dimers and tetramers. A, SDS-PAGE of purified ySMN·Gemin2 complex. B, SEC-MALS analysis indicates a distribution of Mw values from 110 to 180 kDa and a larger than expected Stokes radius based on elution volumes of globular standards. C, SE analysis indicates a dimer-tetramer equilibrium of SMN·Gemin2 heterodimers, with Kd = 1.0 ± 0.9 μm. The Kd was determined from a global fit of three concentrations and three rotor speeds (Table 3). One concentration is shown here.
