TABLE 2.

SAXS analyses of SMN·Gemin2 complexes

R_g and D_max were derived from the inverse Fourier transform as implemented in the program GNOM (32). Results are in agreement with classical Guinier analyses.

Complex	Mcalc	Concentration	Rg	Dmax	Mass by I(0)
	kDa	μm	Å	Å	kDa
hSMN(26–51)·Gemin2	33.7	504	28.1	97	39.1
		252	27.5	94	38.4
		202	27.2	91	37.9
		160	28.1	94	38.5
hSMN(14–156)·Gemin2	46.6	275	40.2	139	46.1
		138	36.8	126	48.6
		69	35.5	121	58.8
hSMN(14–209)·Gemin2	52.3	176	43.7	147	59.9
		88	43.5	146	53.1
		23	44.6	146	59.9
hSMNΔ5·Gemin2	58.4	101	89.3	293	434
		77	97.8	311	438
		63	99.0	300	493
		50	93.4	316	525
ySMN·Gemin2	44.7	134	80.8	275	208
		67	82.8	277	174
		34	83.0	276	141
MBP-hSMN(229–294)	48.3	120	65.2	228	491
		107	65.9	232	480
		72	65.4	224	345
		58	67.6	237	298
		47	64.2	218	275