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RapLS20 interacts with the N terminus of the repressor containing the DNA binding motif. A, graphical representation of the repressor protein and its derivatives used for the translocation assay. B, top, heterologous co-expression of GFP::RapLS20 with DivIVA fusions of RapLS20, Rco, and C- or N-terminally truncated versions of Rco. Bottom, heterologous co-expression of GFP::ΔC-Rco with DivIVA fusions of RapLS20, Rco, and C- or N-terminally truncated versions of Rco. Scale bar, 2 μm.
