FIGURE 10.

Inhibition of P. falciparum growth by PvTRAg38. Parasite culture at late schizont stage was incubated with PvTRAg38, PvTRAg53.7, PfTryThrA, and other controls. After 40 h, parasitemia was determined by ethidium bromide staining measured by flow cytometry for treated cultures as compared with the control. A, representative dot plots showing percent infection. Panel a, unstained erythrocytes; panel b, uninfected erythrocytes; panel c, infected erythrocytes; panel d, infected erythrocytes treated with PBS; panel e, infected erythrocytes treated with PvTRAg38; panel f, infected erythrocytes treated with PfTryThrA; panel g, infected erythrocytes treated with PvTRAg53.7. B, bar graph shows the percentage of parasite growth inhibition in a dose-dependent manner. The data shown are the means ± S.D. for two triplicate experiments.