FIGURE 4.

Erythrocyte binding activity of PvTRAg38 and its peptides by flow cytometry. A, representative dot plots for binding of PvTRAg38 and its fragments/peptides to human erythrocytes. Normal human RBCs (∼1 million) were incubated with 1 μm of recombinant proteins or synthetic peptides and then labeled with an anti-pentahistidine mAb Alexa Fluor 647 conjugate. PvTRAg38 and its fragments/peptides bound to erythrocytes are shown as red dots. The number in the red box refers to percentage of erythrocyte bound by proteins or peptides respective to total erythrocyte population. Thioredoxin and PvTRAg53.7 were taken as negative control. B, bar diagram shows the percent binding of PvTRAg38 or its fragments/peptides with the erythrocytes. The data shown are the means ± S.D. of at least two independent experiments.