FIGURE 3.

Mapping of the region in the Tcf7l1 protein responsible for Jmjd6 interaction. A, domain structure, the construction of the deletion mutants of Tcf7l1 used in the study, and their binding affinities to Jmjd6 after co-IP assays. +++, very strong binding; ++, strong binding; +, weak binding; ±, trace or no binding. B and C, Tcf7l1 deletion mutants exhibited different binding affinities to Jmjd6 as shown by co-IP assays. IB, immunoblot. MT, myc tag. D, immunofluorescence showed that the aa 1–323 region of Tcf7l1 primarily distributed in the cytosol, whereas addition of an NLS to the region relocalized it into the nucleus. E, addition of an NLS to the 1–323 region of Tcf7l1 did not increase its binding affinity to Jmjd6.