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. 2015 Jul 7;290(33):20284–20294. doi: 10.1074/jbc.M115.658625

FIGURE 1.

FIGURE 1.

miR-184 is regulated according to glucose metabolism. A, qRT-PCR analysis of relative miR-184 expression in isolated pancreatic islets (Isl), pituitary (Pit), adrenal gland (Adr), and thyroid (Thy) from 10-week-old C57BL/6 mice. AU, arbitrary units. B, qRT-PCR analysis of relative miR-184 expression in several pancreatic cell lines including MIN6, Panc1, DanG, and MiaPaca. C, qRT-PCR analysis of miR-184 in isolated islets of 10-week-old WT mice on either a normal chow (Ch) or ketogenic (Keto) diet for 75 days (n = 4). Random blood glucose levels were decreased after 1 day on ketogenic diet. Results are presented as mean ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001. D, qRT-PCR analysis of miR-184 in isolated islets of 10-week-old WT mice on either normal chow or ketogenic diets for 25 days. Mice on the ketogenic diet were reverted to normal chow for 0–48 h (n = 4). n.s., not significant. E, blood glucose levels during a glucose tolerance test (GTT) from 10-week-old C57BL/6 mice on normal chow (Chow), ketogenic diet (Keto), and normal chow after 25 days on ketogenic diet (Keto + re-feed) (n = 4). F, blood glucose levels during an insulin tolerance test (ITT) from 10-week-old C57BL/6 mice on normal chow (Chow), ketogenic diet (Keto), and normal chow after 25 days on ketogenic diet (Keto + re-feed) (n = 4). G, qRT-PCR analysis of miR-184 and miR-375 in isolated islets of 10 weeks old WT mice fasted for 24 h. Fed and fasted blood glucose and insulin parameters measured before sacrifice (n = 4). H, qRT-PCR analysis of miR-184 and miR-375 after treating MIN6 cells with either 100 nm insulin or 20 nm of exendin-4 (Ex4) for 48 h (n = 3–4). I, qRT-PCR analysis of miR-184 in isolated islets of 10-week-old WT mice on either normal chow or a low calorie diet for 25 days (n = 4). J, qRT-PCR analysis of miR-184 in Drosophila after sucrose administration and after fasting and re-feeding. After a 16 h starvation period, flies were fed with food in which the only caloric supplement is sucrose (5%). Fly heads were collected from flies re-fed for 0, 6, 12, and 18 h of sugar administration.