TABLE 2.
Primer list
| Primer | Sequence |
|---|---|
| coliDAf | GTTAGGTGGTCGTCCTACGCTACCG |
| coliDAr | CAGTTTTTCCTGCAATGCCAGCAAG |
| tmadnaa4f | CTTGACTGCAGATCCAATAGATGAACTCATAGAGATCG |
| tmadnaa4r | CATGTCTCGAGCAGACCGCTTCTGCGTTCTG |
| SUE260 | ATCCCATGGCCCGGGCCGTGGATTCTAC |
| SUE261 | CTTATGCATGAAGATCAACATTCTTGATCACG |
| DnaAI-f | CCTGTACGCGCCAAACGCGTTTGTCCTCGATTGGG |
| ori1 | ATCGCACTGCCCTGTGG |
| orir1tmafb | GGATCCGGCTTTTAAGATCAAC |
| R1tma12r | AACCTACCACCCAGTGCGATCCTAATAAG |
| R1tma12rb | TTAAACCTACCACCGCAGTGCGATCCTAATAAG |
| R1tma12rc | TAAACCTACCACCGGCAGTGCGATCCTAATAAG |
| R1tma12rd | AAACCTACCACCGGGCAGTGCGATCCTAATAAG |
| R4tmafb | GATCGCACGATCTGTATACTTATTTG |
| R4tma12rb | GGTGGTAGGTTTTGTCAGGAAGCTTG |
| ChDAB-M28-up | GATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGGGTGGTAGGTTTCAAGGA |
| ChDAB-M28-low | TCCTTGAAACCTACCACCCAGTGCGATC |
| tet-oriC f | GGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAATGCGG |
| tet-oriC r | ATAGAACAGATCTCTAAATAAATAGATCTTCTTTTTAATACCCAGGATCAGGTCGAGGTGGCCCGGCTCCATGCACCGCG |
| pSA4 | GCTCTGCCTGATGCCCAGTTGTGTAGGCTGGAGCTGCTTC |
| pSA5 | GACGGGTGTGGTCGCCATGACATATGAATATCCTCCTTA |
| pSA6 | TCATGGCGACCACACCCGTCCTGTGGATCCTCTACGCCGG |
| pSA7 | ATCGATGATAGCTGTCAAACATGAGAATTCTTGAAGACGA |
| pSA8 | TTTGACAGCTTATCATCGATCCGAGTGCATCCACTTCTTT |
| pRS1 | TGTTCACCCATACGCGCCGCGGCCATCGCGGCCTCGGTGC |
| pRS2 | GGTCTGACGTTTCCACTTCGCCAGTGAATGAACCACTTCGCATATGAATATCCTCCTTA |
| R4inv-f | CTACCGGTTGATCCAAGCTTCCTGACAGAGTGTGGATAAGTAGATCGCACGATCTGTATACTTATTTG |
| R4inv-r | CAAATAAGTATACAGATCGTGCGATCTACTTATCCACACTCTGTCAGGAAGCTTGGATCAACCGGTAG |