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. 2015 Jun 16;290(33):20336–20347. doi: 10.1074/jbc.M115.639849

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Regulation of NF-κB-dependent gene expression by Ser-316 phosphorylation. A, a short list of NF-κB-dependent genes that are down-regulated by S316A-p65 mutant. These genes encode proteins that have a broad range of functions. B, quantitative PCR analysis showing that TTLL2, USP28, SLC32A1, NKG7, and TRIM73 were induced by the overexpression of wtp65 as compared with control cells. However, this induction was greatly decreased after S316A-p65 mutation. The data represent the mean ± S.D. from three independent experiments. *, p < 0.01 versus control (Ctrl) group; #, p < 0.01 versus wt group.