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In vivo oxidation of PerRSA. Oxidation of PerRSA from S. aureus cells (LS0166) grown aerobically in MLMM supplemented with no metal ion (A), 50 μm FeSO4 (B), 50 μm MnCl2 (C), or both 50 μm FeSO4 and 50 μm MnCl2 (D). PerRSA-FLAG proteins were recovered by immunoprecipitation from S. aureus cells treated with no H2O2 or 100 μm H2O2 for ∼2 min, and analyzed by MALDI-TOF MS after SDS-PAGE separation and in-gel tryptic digestion.
