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. 2015 Jul 1;290(33):20374–20386. doi: 10.1074/jbc.M115.664961

FIGURE 6.

FIGURE 6.

In vivo repressor activities of PerRSA under oxygen-limited conditions. A, repressor activities and H2O2 sensing abilities of PerRBS and PerRSA under oxygen-limited conditions. S. aureus cells expressing no PerR (LS0124), PerRBS-FLAG (LS0114), or PerRSA-FLAG (LS0111) were grown in LB medium under oxygen-limited conditions, and treated with 100 μm H2O2 (+H2O2) for 30 min or not (−H2O2). Repressor activities of PerR proteins were measured using S. aureus PmrgA-lacZ reporter fusions. B, metal-dependent repressor activities of PerRSA under oxygen-limited conditions. S. aureus cells expressing PerRSA-FLAG (LS0111) were grown in MLMM supplemented with no metal ion, 10 μm FeSO4, 10 μm MnCl2, or both 10 μm FeSO4 and 10 μm MnCl2 under oxygen-limited conditions, and treated with 100 μm H2O2 (+H2O2) for 30 min or not (−H2O2). Repressor activities were measured using S. aureus PmrgA-lacZ reporter fusion. A and B, repressor activities were expressed as Miller units of β-galactosidase activity (values represent the mean ± S.D. from three separate experiments). Statistical analysis was performed with a Student's t test (*, p < 0.05; NS, not significant).