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. 2015 Jul 1;290(33):20374–20386. doi: 10.1074/jbc.M115.664961

FIGURE 7.

FIGURE 7.

Effects of PerR activity on H2O2 resistance and virulence of S. aureus. A, effects of PerR activity on the survival of S. aureus in the absence or presence of H2O2. S. aureus cells (3 μl of A600 = 1 culture, with indicated dilutions) expressing no PerR (LS0093), PerRBS-FLAG (LS0134), or PerRSA-FLAG (LS0088) were spotted on LB-agar plate containing no H2O2 or 200 μm H2O2, and grown under aerobic conditions at 37 °C. Note that fresh LB agar plate shielded from light was used to prevent the generation of H2O2 by photochemical reactions (41). B, effects of PerR activity on the expression of KatA. S. aureus cells expressing no PerR (LS0093), PerRBS-FLAG (LS0134), or PerRSA-FLAG (LS0088) were grown in LB medium under either aerobic or oxygen-limited conditions, and treated with 100 μm H2O2 or not. Catalase activity staining was performed after separation of cell extract (10 μg protein) on native PAGE gel. C, effects of PerR activity on the pathogenicity of S. aureus using C. elegans as a model host. C. elegans killing was presented as Kaplan-Meier survival plots of worms fed with E. coli OP50 (negative control) (n = 86), S. aureus strain expressing no PerR (LS0093) (n = 86), PerRBS-FLAG (LS0134) (n = 77), or PerRSA-FLAG (LS0088) (n = 84).