TABLE 1.
Strains used in this study
| Strains | Relevant genotype and feature | Source |
|---|---|---|
| S. aureus | ||
| Newman | Wild type, human clinical isolate | NCTC |
| RN451 | RN450 lysogenic for Φ11 | NARSA |
| RN4220 | Restriction-deficient transformation recipient | NARSA |
| LS0085 | Newman perR::cat | This study |
| LS0088 | LS0085 pLL29::perRSA-FLAG | This study |
| LS0093 | LS0085 pLL29 | This study |
| LS0106 | Newman pCN33::PmrgA-lacZ | This study |
| LS0107 | Newman pCN33::PkatA-lacZ | This study |
| LS0111 | LS0085 pLL29::perRSA-FLAG pCN33::PmrgA-lacZ | This study |
| LS0114 | LS0085 pLL29::perRBS-FLAG pCN33::PmrgA-lacZ | This study |
| LS0124 | LS0085 pLL29 pCN33::PmrgA-lacZ | This study |
| LS0134 | LS0085 pLL29:: perRBS-FLAG | This study |
| LS0166 | LS0085 pCN48::perRSA-FLAG | This study |
| LS0241 | LS0085 pLL29::perRSA-FLAG pCN33::PkatA-lacZ | This study |
| LS0242 | LS0085 pLL29::perRSA (H43A)-FLAG pCN33::PkatA-lacZ | This study |
| LS0243 | LS0085 pLL29::perRSA (H97A)-FLAG pCN33::PkatA-lacZ | This study |
| LS0244 | LS0085 pLL29::perRSA (C102S)-FLAG pCN33::PkatA-lacZ | This study |
| LS0245 | LS0085 pLL29 pCN33::PkatA-lacZ | This study |
| LS0304 | LS0085 pLL29::perRSA (D91A)-FLAG pCN33::PkatA-lacZ | This study |
| LS0305 | LS0085 pLL29::perRSA (H99A)-FLAG pCN33::PkatA-lacZ | This study |
| LS0306 | LS0085 pLL29::perRSA (C105S)-FLAG pCN33::PkatA-lacZ | This study |
| LS0307 | LS0085 pLL29::perRSA (D110A)-FLAG pCN33::PkatA-lacZ | This study |
| LS0308 | LS0085 pLL29::perRSA (C142S)-FLAG pCN33::PkatA-lacZ | This study |
| LS0309 | LS0085 pLL29::perRSA (C145S)-FLAG pCN33::PkatA-lacZ | This study |
| B. subtilis | ||
| HB9703 | perR::tet | Ref. 15 |
| HB9738 | HB9703 amyE::perRBS-FLAG SPβC2Δ2::Tn917::Φ (PmrgA-cat-lacZ) | Ref. 15 |
| LB1530 | HB9703 amyE::perRSA-FLAG SPβC2Δ2::Tn917::Φ (PmrgA-cat-lacZ) | This study |
| LB1532 | HB9703 amyE::spc SPβC2Δ2::Tn917::Φ (PmrgA-cat-lacZ) | This study |
| E. coli | ||
| HE9501 | BL21 (DE3) pLysS pET-16b::perRBS | Ref. 15 |
| LE0003 | BL21 (DE3) pLysS pET-16b::perRSA | This study |
| LE0031 | BL21 (DE3) pLysS pET-16b::perRSA (H43A) | This study |
| LE0032 | BL21 (DE3) pLysS pET-16b::perRSA (D91A) | This study |
| LE0033 | BL21 (DE3) pLysS pET-16b::perRSA (H97A) | This study |
| LE0034 | BL21 (DE3) pLysS pET-16b::perRSA (H99A) | This study |
| LE0035 | BL21 (DE3) pLysS pET-16b::perRSA (C102S) | This study |
| LE0036 | BL21 (DE3) pLysS pET-16b::perRSA (C105S) | This study |
| LE0037 | BL21 (DE3) pLysS pET-16b::perRSA (D110A) | This study |
| LE0038 | BL21 (DE3) pLysS pET-16b::perRSA (C142S) | This study |
| LE0039 | BL21 (DE3) pLysS pET-16b::perRSA (C145S) | This study |
| LE2302 | BL21 (DE3) pLysS pET-11a::oxyREC | This study |