FIGURE 2.

RGC-32 deficiency reduced the expression of lipogenic genes. A, mRNA expression of fatty acid-, triglyceride synthesis-, and β-oxidation-related genes in the livers of HFD-fed WT and RGC32^−/− mice (n = 6). B–E, protein expression of SREBP-1c, FAS, and SCD1 in livers from WT and RGC32^−/− mice fed an HFD (B and C) or normal chow (D and E) were detected by Western blot analysis and normalized to α-tubulin (n = 6). *, p < 0.05 and **, p < 0.01 compared with WT chow groups; #, p < 0.05 and ##, p < 0.01 compared with WT HFD groups.