RGC-32 directly regulated hepatic SREBP-1c and SCD1 expression. Mouse primary hepatocytes were transduced with Ad-GFP, Ad-shRGC32, or Ad-RGC32 for 48 h. A, qPCR was performed to detect mRNA expression of RGC-32, SREBP-1c, and SCD1 as indicated. B and C, RGC-32, SREBP-1c, and SCD1 protein expression was detected by Western blot analysis and normalized to α-tubulin. All results are representative of at least three independent experiments. *, p < 0.05 and **, p < 0.01 compared with Ad-GFP groups.