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. 2015 Jul 1;290(33):20387–20395. doi: 10.1074/jbc.M114.630186

FIGURE 8.

FIGURE 8.

RGC-32 regulated hepatic SCD1 expression in an SREBP-1c-dependent manner. A, mouse primary hepatocytes were transduced with Ad-GFP, Ad-shRGC32, or Ad-RGC32 for 24, followed by transfection of different SCD1 promoter constructs, as indicated, for 48 h. A luciferase assay was performed. Luciferase activity was normalized to Renilla activity. B and C, mouse primary hepatocytes were transduced with Ad-GFP, Ad-shRGC32, or Ad-RGC32 for 48 h, and a ChIP assay was performed. SREBP-1c binding enrichment was detected by RT-PCR (B) and qPCR (C). D and E, mouse primary hepatocytes were transfected with scramble (siCtrl) or SREBP-1c siRNA (siSREBP-1c) for 24 h and then transduced with Ad-GFP or Ad-RGC32 for 48 h as indicated. SCD1, RGC-32, and SREBP-1c were detected by Western blot analysis and normalized to α-tubulin. All results are representative of at least three independent experiments. *, p < 0.05 and **, p < 0.01 compared with Ad-GFP groups; ##, p < 0.01 compared with siCtrl + AdRGC32 groups.