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. 2015 Jul 1;290(33):20387–20395. doi: 10.1074/jbc.M114.630186

FIGURE 9.

FIGURE 9.

RGC-32 regulated hepatic SREBP-1c expression through activating LXR. A and B, mouse primary hepatocytes were transduced with Ad-GFP, Ad-shRGC32, or Ad-RGC32 for 48 h. LXR-α and RGC-32 were detected by Western blot analysis and normalized to α-tubulin. C and D, mouse primary hepatocytes were preincubated with 100 μm arachidonic acid (AA) or vehicle for 1 h and then transduced with Ad-GFP or Ad-RGC32 for 48 h. SREBP-1c and RGC-32 were detected by Western blot analysis and normalized to α-tubulin. E and F, mouse primary hepatocytes were transduced with Ad-GFP, Ad-shRGC32, or Ad-RGC32 for 48 h, and a ChIP assay was performed. LXR-α binding enrichment was detected by RT-PCR (E) and qPCR (F). All results are representative of at least three independent experiments. *, p < 0.05 and **, p < 0.01 compared with Ad-GFP groups; ##, p < 0.01 compared with Ad-RGC32 groups.