FIGURE 2.
Fly mutants of gabat exhibit overt signals of metabolic stress. a, mutants of gabat cannot survive on sucrose food. When fed a sucrose-only diet, males of our gabatPL and gabatf strains die after 7 days. Fisher's exact test, ***, p < 0.0001. b, GABAT metabolic phenotype is rescued on regular food. When males of the gabatf strain are transferred to a regular food diet, they no longer exhibit overt signals of metabolic stress. Fisher's exact test, p = 0.24. c, metabolic phenotype of gabatf is rescued by driving gabat expression in either neurons or glia. A one-way ANOVA between subjects was conducted to compare the effect of genotype on lethality in gabatf flies carrying UAS-GABATvH (U) driven by GAL4 drivers in glial cells using Repo-GAL4 (Repo) or neurons using ELAV-GAL4 (Elav). The following lines were used as a negative controls: gabatf; gabatf,Elav; gabatf,Repo; and gabatf,U. There was a significant effect of genotype on lethality at the p < 0.05 level for the four groups (F(6) = 30.8, p < 0.0001). Lines with different letter values (A and B) are statistically different as determined by the Tukey post hoc HSD test.