FIGURE 7.

GABAT regulates sleep independently of glutamate. When we fed gabat^f mutant (red) and wild-type (blue) flies a sucrose diet enhanced with the amino acids that can be metabolized into glutamate and can rescue the metabolic phenotype (Fig. 5, b and c; Table 2), we found that the sleep phenotype of gabat^f cannot be rescued, suggesting that GABAT regulates sleep independently of metabolic homeostasis. We used a one-way ANOVA between subjects to compare total sleep time (minutes) of gabat^f mutant and wild-type flies fed sucrose, alanine, glutamate, glutamine, leucine, phenylalanine, tyrosine, and valine. There was a significant effect of the food treatment on total sleep at the p < 0.05 level for the 13 groups, F(8) = 9.68, p < 0.0001. We used Tukey post hoc comparisons to compute the groups that were statistically significant: different levels (A–C) indicate statistical significance. None of the amino acids reduced sleep relative to the negative controls.