Pressure inhibits heat-induced PrP oligomerization.
a, oligomer molecular weight estimation (expressed as the number of equivalent monomers) by multiwavelength light scattering coupled with size exclusion chromatography. b, size exclusion chromatograms obtained after a 1-h incubation (55 °C) of PrP at atmospheric pressure (black line), 50 MPa (blue line), and 150 MPa (red line). The PrP species corresponding to each peak are indicated. a.u., arbitrary units.