FIGURE 2.

Pressure induces PrP oligomer dissociation. Overlay of size exclusion chromatograms of purified O0 (top), O1 (middle), and O3 (bottom) PrP oligomers untreated (at 15 °C for 1 h; blue line) or after a cycle (1 h) of compression (550 MPa)/decompression at 15 °C (red line). The profile of monomeric PrP (black line) is shown in each panel to estimate the amount of resolubilized PrP rescued from each oligomeric form after pressure treatment. The PrP species corresponding to each peak are indicated. a.u., arbitrary units.