FIGURE 7.

Kinetics of the pressure- and temperature-induced dissociation of the O1 PrP oligomer. Temperature (a) and pressure (b) dependence of the pressure-induced O1 structural kinetics. a, pressure jumps (from 0.1 to 300 MPa) were performed at different temperatures: 15 °C (black line), 25 °C (blue line), 37 °C (red line), and 45 °C (green line). b, pressure jumps (at 37 °C) were performed starting at 0.1 MPa to obtain different final pressures: 200 MPa (black line), 250 MPa (blue line), 300 MPa (red line), and 350 MPa (green line). c, temperature dependence of the temperature-induced O1 structural kinetics. Rapid temperature increases of different magnitudes (starting at 15 °C and always at 0.1 MPa) were performed to reach different final temperatures: 70 °C (black line), 75 °C (blue line), 80 °C (red line), and 85 °C (green line). Insets show the pressure and temperature dependence of the observed rate constants (see “Experimental Procedures” for further details). Solid lines, linear fits to the data. a.u., arbitrary units.