FIGURE 5.

DHA induces GPR120. A and B, total mRNA was extracted from DHA-treated (A) and GW9058-treated (B) C2C12 cells. RT-PCR was performed using GPR120-specific primers. PCR products were visualized under ultraviolet light. β-Actin was used as a positive control. The results are representative of four independent experiments. *, p < 0.05 versus basal conditions. C, L6 myotubes were transiently transfected with GPR120 siRNA and incubated with DHA for 1 h. Uptake of 2-DG was assayed. *, p < 0.05 compared with control. D, C2C12 cells were transfected with GPR120 siRNA for 2 days and then treated with 100 μm DHA. Green fluorescence was detected and analyzed.