FIGURE 6.

DHA activates AMPK and stimulates glucose uptake in primary cultured myoblasts. A, primary cultured myoblasts were stimulated with DHA for 1 h in the presence of compound C. Cell lysates were analyzed by Western blotting with antibodies against phospho-ACC, with ACC as the control. The results are representative of four independent experiments. *, p < 0.05 versus basal conditions. B, differentiated primary cultured myoblasts were incubated with DHA for 1 h in the presence of compound C (2 μm) or STO-609 (2 μm) and were assayed for glucose uptake. C, for detecting Ca²⁺, the cells were pretreated with fluo-3 AM (5 μm) for 45 min. Ca²⁺ response after DHA treatment was measured using confocal microscopy.