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. 2015 Jun 24;290(33):20477–20487. doi: 10.1074/jbc.M115.650531

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Relative distribution of GFP-RyR2 clusters and the tubular system. A, the tubular membrane system in live GFP-RyR2 ventricular myocytes (n = 27) was labeled with di-8-ANEPPS and visualized with confocal fluorescence imaging. B, GFP-RyR2 cluster fluorescence signals of the same cell. C, merged image of the di-8-ANEPPS and GFP-RyR2 signals. The panels at the bottom show co-localization of GFP-RyR2 clusters with transverse tubules (panel a), no co-localization of GFP-RyR2 clusters with longitudinal tubules (panel b), and co-localization of a few GFP-RyR2 clusters with longitudinal tubules located near the perinuclear region (panel c).