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Co-localization of Ca2+ sparks and GFP-RyR2 clusters in live ventricular myocytes. Ventricular myocytes (n = 101) isolated from GFP-RyR2 mice were loaded with the fluorescent Ca2+ dye Rhod-2/AM. The Rhod-2 fluorescence signals (top panel) and the GFP-RyR2 cluster fluorescence signals (middle panel) were simultaneously recorded using confocal line scanning. The merged image of Ca2+ sparks and GFP-RyR2 clusters is shown (bottom panel).
