TABLE 2.
Primers for quantitative PCR of bacterial 16S rRNA genes
Gene | Forward primera | Reverse primer |
---|---|---|
Clostridium coccoides group | AAATGACGGTACCTGACTAA | CTTTGAGTTTCATTCTTGCGAA |
C. leptum subgroup | GCACAAGCAGTGGAGT | CTTCCTCCGTTTTGTCAA |
Bifidobacterium | CTCCTGGAAACGGGTGG | GGTGTTCTTCCCGATATCTACA |
Atopobium cluster | GGGTTGAGAGACCGACC | CGGRGCTTCTTCTGCAGG |
B. fragilis group | AYAGCCTTTCGAAAGRAAGAT | CCAGTATCAACTGCAATTTTA |
Prevotella | CACRGTAAACGATGGATGCC | GGTCGGGTTGCAGACC |
E. cylindroides group | GTGAYGGTAKCTTACCAGA | CTTGCGTGCATACTCCC |
Lactobacillus group | AGCAGTAGGGAATCTTCCA | CACCGCTACACATGGAG |
Total bacteria | GTGCCAGCMGCCGCGGTAA | GACTACCAGGGTATCTAAT |
a Nucleotide base codes used are as follows: Y, C, or T; R, A, or G; K, G, or T; M, A, or C.