FIGURE 8.

XN blocks SREBP processing in vivo. Male 6-week-old C57BL/6J mice received oral administration of vehicle or XN (75 or 150 mg/kg body weight) once daily. The mice had ad libitum access to water and a standard chow diet. After a 3-day treatment, livers were harvested from the mice. A, average mouse body weight for each treatment group during the 4-day experiment. B, total average mouse food intake for each treatment group during the 4-day experiment. C, immunoblotting (IB) analysis of liver extracts with anti-SREBP-1 (H-160) or anti-β-actin antibodies. D and E, real time quantitative PCR analysis of gene expression in the liver. Relative mRNA levels were obtained by normalization to 18S rRNA. All data are presented as mean ± S.E. (n = 5). Different superscript letters denote statistical significance (p < 0.05).