Table 1. Quantitative RT-PCR validation of differentially expressed genes.
| Gene | LW-short VS LW-long |
QP-short VS LW-short |
QP-long VS LW-short |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RNA-seq | P value | qPCR | P value | RNA-seq | P value | qPCR | P value | RNA-seq | P value | qPCR | P value | |
| GGTA1 | −1.95 | <0.01 | −2.17 | <0.01 | 2.41 | <0.01 | 1.33 | 0.08 | 1.46 | <0.01 | 1.61 | <0.01 |
| LIPG | 3.84 | <0.01 | 4.35 | <0.01 | −1.96 | <0.01 | −9.46 | <0.01 | −1.42 | 0.03 | −3.93 | <0.01 |
| CXCL14 | −3.59 | <0.01 | −5.47 | <0.01 | 2.97 | <0.01 | 2.83 | <0.01 | 3.04 | <0.01 | 1.21 | <0.01 |
| APOE | 3.46 | <0.01 | 1.19 | <0.01 | −4.22 | <0.01 | −1.76 | <0.01 | −3.18 | <0.01 | −3.94 | <0.01 |
| RERG | −2.6 | <0.01 | −7.76 | <0.01 | 2.58 | <0.01 | 1.04 | <0.01 | 1.55 | 0.11 | −1.74 | 0.37 |
| HSPA4 | −1.47 | 0.01 | −2.29 | <0.01 | 1.43 | 0.01 | 1.65 | 0.02 | 1.11 | 0.31 | 1.05 | 0.27 |
| IGFBP2 | −1.53 | 0.02 | −2.62 | 0.03 | 1.24 | 0.03 | 1.06 | 0.09 | −1.84 | <0.01 | −4.33 | 0.04 |
| TNFRSF12A | −2.07 | <0.01 | −3.52 | 0.03 | 2.08 | <0.01 | −2.14 | <0.01 | 1.75 | <0.01 | −2.51 | 0.65 |
| RAMP1 | −1.08 | 0.07 | −6.32 | 0.04 | −1.46 | <0.01 | −31.96 | <0.001 | −4.14 | <0.01 | −7.96 | <0.01 |
| HBB | −2.74 | <0.01 | −1.62 | 0.05 | 1.91 | 0.02 | 1.67 | 0.05 | −1.4 | 0.01 | −1.95 | 0.07 |
| HSP70 | −18.97 | 0.31 | −12.06 | 0.04 | 12.22 | 0.01 | 2.25 | 0.04 | 5.23 | <0.01 | 1.32 | 0.43 |
| SERPINA3-2 | −4.03 | 0.01 | −1.47 | 0.07 | 4.06 | 0.03 | 2.86 | 0.04 | 3.12 | 0.05 | −1.46 | 0.33 |
| MMP1 | −4.3 | 0.25 | −12.25 | <0.01 | 3.46 | <0.01 | 3.4 | <0.001 | 1.45 | 0.08 | 1.56 | 0.04 |
qRT-PCR-based validation of GGTA1, LIPG, CXCL14, APOE, RERG, HSPA4, IGFBP2, TNFRSF12A, RAMP1, HBB, HSP70, SERPINA3-2, MMP1, CYP2C36 and PGHS-2 expression levels in LN, LL, QS and QL.