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. 2015 Aug 13;6:8010. doi: 10.1038/ncomms9010

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(a) MP1 and DC1 subsets were isolated from uninfected (uninf) and C. rodentium-infected (inf) CD115^GFP animals. Cytokine mRNA expression was analysed by qPCR. Data are given as mean±s.d. (n=5–7). *P<0.05; **P<0.01; ***P<0.001; NS, not significant by Dunn's test. (b) Purified MP1 and DC1 subsets (1 × 10⁶ cells per ml) were cultured for 24 h without stimulation. Cytokines in the culture supernatant were analysed by ELISA. Data are given as mean±s.d. (n=4–6). (c) LPMCs were isolated from C. rodentium-infected CCR2^WT and CCR2^DTR mice. 2 × 10⁶ cells per ml LPMCs were cultured for 24 h without stimulation. Cytokines in the culture supernatant were analysed by ELISA. Data are given as mean±s.d. (n=3, representative of three independent experiments). *** P<0.001; NS, not significant by Student's t-test.