Table 2. Proteins exclusively visualized in the CSF of JEV infected patients, identified by MS/MS analysis of excised spots.
| Sr.
No |
Spot
No. |
Protein ID.
(Accession) a |
Matched peptides | Ion
score |
%
coverage |
Mowse
Score b |
MW
(theor/ obs) |
pI
(theor/ obs) |
|---|---|---|---|---|---|---|---|---|
| 1. | JE-1 | Serum albumin
(NP_000468) |
K.LVNEVTEFAK.T
K.SLHTLFGDKLCTVATLR.E K.KYLYEIAR.R K.YLYEIAR.R |
30
56 56 44 |
59 | 1480 | 71/70 | 5.9/5.5 |
| 2. | JE-2 | Vitamin D-
binding protein (NP_000574) |
R.KFPSGTFEQVSQLVK.E
K.EVVSLTEACCAEGADPDCYDTR.T K.SCESNSPFPVHPGTAECCTKEGLER.K K.HQPQEFPTYVEPTNDEICEAFR.K K.HLSLLTTLSNR.V |
77
89 142 74 97 |
31 | 614 | 54/60 | 5.4/5.4 |
| 3. | JE-3 | Fibrinogen
gamma chain (NP_000500) |
R.DNCCILDER.F
R.YLQEIYNSNNQK.I K.QSGLYFIKPLK.A K.IHLISTQSAIPYALR.V K.IHLISTQSAIPYALR.V |
34
72 50 90 36 |
24 | 595 | 52/54 | 5.37/5.9 |
| 4. | JE-4 | Fibrinogen beta
chain (NP_005132) |
R.GHRPLDKK.R
K.HQLYIDETVNSNIPTNLR.V R.SILENLR.S R.TPCTVSCNIPVVSGKECEEIIR.K R.QDGSVDFGR.K |
22
69 21 49 40 |
35 | 286 | 56/60 | 8.54/6.9 |
| 5. | JE-5 | Fibrinogen beta
chain (NP_005132) |
R.EEAPSLRPAPPPISGGGYR.A
K.HQLYIDETVNSNIPTNLR.V R.SILENLR.S R.QDGSVDFGR.K |
19
78 26 23 |
22 | 199 | 56/60 | 8.54/7.2 |
| 6. | JE-6 | Fibrinogen beta
chain (NP_005132) |
R.GHRPLDK.K
R.EEAPSLRPAPPPISGGGYR.A K.HQLYIDETVNSNIPTNLR.V R.SILENLR.S R.TPCTVSCNIPVVSGKECEEIIR.K |
10
19 68 26 42 |
25 | 232 | 56/54 | 8.54/7.7 |
| 7. | JE-7 | Complement C4b
(NP_001002029) |
R.EAPKVVEEQESR.V
R.VHYTVCIWR.N R.YVSHFETEGPHVLLYFDSVPTSR.E R.ECVGFEAVQEVPVGLVQPASATLYDYYNPERR.C R.RCSVFYGAPSK.S |
88
61 115 55 18 |
17 | 864 | 194/31 | 6.8/6.8 |
| 8. | JE-8 | Actin,
cytoplasmic-1 (NP_001092) |
K.AGFAGDDAPR.A
R.AVFPSIVGRPR.H K.DSYVGDEAQSKR.G K.DSYVGDEAQSKR.G |
33
53 52 120 |
48 | 1070 | 42/50 | 5.29/5.4 |
| 9. | JE-9 | Complement C4b
(NP_001002029) |
R.EAPKVVEEQESR.V
R.VHYTVCIWR.N R.CSVFYGAPSK.S R.LLATLCSAEVCQCAEGKCPR.Q R.GLQDEDGYR.M |
88
60 54 142 41 |
12 | 634 | 194/45 | 6.8/6.0 |
| 10. | JE-10 | Complement C3
(NP_000055) |
K.VYAYYNLEESCTR.F
K.ACEPGVDYVYKTR.L K.SGSDEVQVGQQR.T K.SDDKVTLEER.L |
90
54 49 34 |
11 | 334 | 188/45 | 6.02/4.8 |
aNCBI accession number of identified proteins is mentioned.
bMS/MS data of 3 peptides for each spot was searched against NCBI database in the taxonomy group of Homo sapiens using Mascot tool.