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. 2015 Aug 14;5:13146. doi: 10.1038/srep13146

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Copyright © 2015, Macmillan Publishers Limited

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C57BL/6 mice (n = 5 mice/group) were injected i.p. with PBS, alum mixed with PBS at 1:2 ratio, IL-33 or IL-33 mixed with 8μg mouse genomic DNA in combination with NP-CGG then boosted i.p. on day 14 with PBS/NP-CGG. Antigen specific IgM, IgG1and IgE serum titers were quantified via ELISA. Data are representative of two independent experiments. *p < 0.05 and ***p < 0.001 alum compared to IL-33/DNA groups (Two-way ANOVA with Bonferroni multiple comparison test). ##p < 0.01 IL-33 compared to IL-33/DNA groups (Two-way ANOVA with Bonferroni multiple comparison test).