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. 2015 Aug 15;13:92. doi: 10.1186/s12958-015-0093-1

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© Plante et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Isolation and identification of ram BSP proteins. a Chromatogram of ram SP proteins (150 mg) on gelatin-agarose column. The gelatin-adsorbed (GA) proteins (tubes 55–61) were pooled, desalted and freeze-dried. b SDS-PAGE of ram SP proteins (20 μg) and of GA fraction (15 μg). c Immunoblot analysis of ram BSP proteins using the polyclonal antibodies directed against ram BSP proteins. Ram SP proteins (2 μg) and GA proteins (200 ng). d Specificity of the polyclonal antibodies directed against ram BSP proteins. SP, ram seminal plasma proteins (300 ng) and SM, skimmed milk proteins (10 μg)