Fig. 5.

Gel filtration analysis of stallion SP proteins and interaction between stallion BSP proteins and milk proteins. a Gel filtration chromatogram of 1.75 mg of stallion SP proteins alone (open circles) or after incubation with skimmed milk (solid circles). b Immunoblot analysis of fractions from the elution of stallion SP proteins alone. Following the elution, aliquots equivalent to 6 % of tubes from five fractions (1, tubes 43–51; 2, tubes 52–74; 3, tubes 75–100; 4, tubes 101–126 and 5, tubes 127–141). c Immunoblot analysis of pooled fractions from the elution of stallion SP proteins incubated with skimmed milk. SP, stallion seminal plasma proteins (300 ng). Following elution, fractions were pooled in seven fractions (I, tubes 43–51; II, tubes 52–70; III, tubes 71–90; IV, tubes 91–100; V, tubes 101–110; VI, tubes 111–120 and VII, tubes 121–148). Aliquots equivalent to 0.3 % of each fraction were precipitated. The experiments were carried out in triplicates and the results of one typical experiment are shown