Fig. 6.

Gel filtration analysis of ram SP proteins and interaction between ram BSP proteins and milk proteins. a Gel filtration chromatogram of 1.75 mg of ram SP proteins alone (open circles) or after incubation with skimmed milk (solid circles). Following the elution, tubes were pooled to provide five fractions for ram SP proteins alone (1, tubes 40–50; 2, tubes 51–80; 3, tubes 81–100; 4, tubes 101–130 and 5, tubes 131–141) or seven fractions for SP incubated with milk (I, tubes 40–50; II, tubes 51–70; III, tubes 71–90; IV, tubes 91–100; V, tubes 101–110; VI, tubes 110–120 and VII, tubes 121–142). Aliquots equivalent to 3 % of each fraction were precipitated and used for immunoblots. b Immunoblot analysis of pooled fractions from the elution of ram SP proteins alone. SP, ram seminal plasma proteins (300 ng). c Immunoblot analysis of pooled fractions from the elution of ram SP proteins incubated with skimmed milk. The experiments were carried out in triplicates and the results of one typical experiment are shown