Figure 2. Prp8 in tri-snRNP.
a, Domain organization of Prp8. The structure of the N-terminal domain (residues 1-884) is unknown. RT, Reverse transcriptase-like domain; X, Thumb/X; L, linker, RH, RNaseH-like; JM, Jab1/MPN domains. b, The large domain of Prp8 is located at the centre of tri-snRNP. The Jab1/MPN domain is bound to Brr2 (ref. 31,32). c, Inset, Loop 1 of U5 snRNA is inserted to the active site cavity and in contact with Dib1. d, Prp8 in the Prp8/Aar2 complex10 is shown with its large domain in the same orientation as in b. In tri-snRNP, the RNaseH domain is inverted while the Jab1/MPN domain in complex with Brr2 is located at the opposite end of the large domain.