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. 2015 Aug 14;15:201. doi: 10.1186/s12870-015-0558-6

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© Singh et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Loss of centromeric cohesion in pans1 male meiocytes during Meiosis I. FISH on male meiotic chromosome spreads using a centromeric repeat probe showing DAPI (blue) and probe (red). Left column: merged images of DAPI and the probe; middle column: probe alone; right column: DAPI. a-c wild type metaphase I. d-i pans1 metaphase I. d-f mild phenotype showing split centromere signal on one chromosome indicated by arrow head. (g-i) strong phenotype showing univalents of one chromosome and split centromere signals on four chromosomes. Scale bar 10 μm