Fig. 3.

Changes in intracellular pH (pH_i) of osteoclasts. The pH_i of primary osteoclasts labeled with the pH-sensitive dye BCECF-AM (12 μM) was monitored along time in order to evaluate the H⁺ secretion. a. Primary osteoclast exhibit rhythmic fluctuations in pH_i after the NH₄Cl prepulse under non-flowing standard HEPES-buffered solution. b. Different patterns of pH_i oscillations are observed in the primary osteoclasts under non-flowing standard HEPES-buffered solution, with variations in period and amplitude in cycles of acidification and alkalinization, in the presence or absence of NH₄Cl prepulse. Each line represents one osteoclast